Artificial Organic Living: Another Quantum Leap
May 23, 2010 1 Comment
Did you ever thought about it? Artificial manipulation of genome and creating the synthetic genome is, for sure a quantum leap in science. This is what scientists at J. Craig Ventor Institute has done. They created first self replicating synthetic bacterial cell made by a process which included the doping of 1.08million base pair chromosomes(all were synthetic) of Micoplasma Mycoid genome into a host cell. The team designed 1078 cassets that were 1078 base pair long. They used yeast assembly system to make genome in three main stages. First stage involved taking 10 DNA cassets at a time to build 10, 110 and 000 bp segments. In second stage it taken 10,000 bp segment 10 at a time to built 100,000 bp segment. And finally all 11,100kb assembled into complete synthetic genome in yeast cells and grown as a yeast artificial genome. The complete syntheticM. mycoidesgenome was isolated from the yeast cell and transplanted intoMycoplasma capricolum recipient that have the genes for its restriction enzymes removed. There were errors initially which were corrected by using semi synthetic genome which result into a viable synthetic cell production. The biggest outcome is self replicating synthetic biological machine which is a great achievement of science. Although there are ethical issues which are opposing this Discovery. Many depicted it as biological grey goo which may lead to a total catastrophe for human. But I don’t think this is going to become such adverse and there are no reasons for such hypothesis at least for now.